Fed blood glucose levels and glucose tolerance following dietary switches in male C57BL/6 mice. Fed blood glucose levels (A) and glucose tolerance (B), determined by area under the curve (AUC), in ad libitum (AL), dietary restriction (DR), AL-DR and DR-AL mice at 1 month, 6 months and 10 months post switch. Groups sharing the same letter within the same timepoint (that is, 1 month, 6 months or 10 months) were not significantly different to one another. Results are reported as mean ± standard error of the mean (SEM), with P <0.05 regarded as statistically significant. In addition, glucose tolerance curves (C-E) are shown for all groups at 1 month, 6 months or 10 months post switch, respectively. N = 7 to 10 per group.