Glucose tolerance in female (F; (A)) and male (M; (B)) mice during double crossover. Data were calculated as the area under the curve (AUC) following a glucose injection and represent means ± SEM from N = 6 to 10 mice/group. Asterisks denote significant differences between groups (*P <0.05; **P <0.001), assessed using one-way analysis of variance (ANOVA). In male AL-DR-AL mice, glucose tolerance was significantly better at the end than at baseline (P <0.001). AL = ad libitum; DR = dietary restriction.