Functional tests performed at different survival time points (3, 17, 20, 23, 26 and 29 months old) in the longevity curve. A) shows the mouse grip strength values in grams. All the available animals were tested at the different ages. B) shows motor coordination. It was determined as the percentage of animals that successfully passed the tightrope test. The fraction of mice passing the test is indicated above the bars. The number of animals tested (n = 12 to 24) varied at the different ages. C) shows the maximal running speed achieved in a VO2max test and that was considered the maximal aerobic workload capacity of the animal (n = 20). Values are shown as mean ± SD. (*) indicates P <0.05, (**) indicates P <0.01 versus the sedentary group at the different ages. Continuous lines show statistically significant differences between the sedentary and the wheel-running groups. VO2max, exercise capacity.