Table 1 Oligonucleotide primer sequences for quantitative PCR assessment of Guinea pig myosin heavy chain mRNA
Gene name | Forward primer (5'–3') | Reverse primer (5'–3') | Amplicon size (bp) |
|---|---|---|---|
MyH1 (MHC IIx) | TTCATCCAAATGCAGGAAAG | TCTTTATCTCAAAAGTCATAAATACAA | 90 |
MyH2 (MHC IIa) | TGTGGAATGACCAGAGCAAG | CCTTTGCAATAGGGTAGGACA | 85 |
MyH4 (MHC IIb) | TCCATCTACTGCTGCAACG | ACTCTGCAGATTTTATTTCCTTG | 93 |
MyH7 (MHC I) | AAGTATCGCAAGGCTCAA | CCTTTCCTTAATTCCAAGC | 129 |