Figure 4

oxa-1::gfp expression pattern. A, B: Transgenic strain AW241 (ouEx609 (pAW318 + rol-6^-)), where pAW318 is a translational GFP fusion of oxa-1 driven by the endogenous promoter. oxa-1 is expressed at a high level throughout the worm in a reticular mitochondrial network pattern characteristic of mitochondrial proteins. A: expression of oxa-1::gfp is particularly strong in body wall muscle cells, where it is excluded from the nuclei (white arrow). B: co-localization of oxa-1::gfp (left hand green image) and Mitotracker Red (central red image) in the merged image (right hand yellow image), confirming expression of oxa-1::gfp in mitochondrial networks. Scale bar, 100 μm. Anterior is to the left in all images.