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Table 1 Primer sets (5′-3′) used for real-time PCR analysis of gene expression

From: Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice

    Gene of interest

GenBank accession #

   Forward primer

    Reverse primer

Methionine adenosyltransferase 1a (Mat1a)

NM_133653

ctgaggcgctctggtgtc

tcctgcatgtactgaactgttacc

Glycine N-methyltransferase (Gnmt)

NM_010321

gctggacgtagcctgtgg

cacgctcatcacgctgaa

S-adenosylhomocysteine hydrolase (Ahcy)

NM_016661

ctgttggggttcacttcctg

acattcagcttgcccaggt

Cystathionine β-synthase (Cbs)

NM_144855

cgcacaggaaggactgcta

agccttcacagccacagc

Cystathionase (Cth)

NM_145953

gagtctggctgagcttcca

cgagggtagctctgtccttc

Betaine homocysteine S-methyltransferase (Bhmt)

NM_016668

acgtggacttcctcattgcagagt

tgctacgggcttaccagatgcttt

5-Methyltetrahydrofolate-homocysteine methyltransferase (5-MeTHF-hmt)

XM_138431

gcagatgtggccagaaaag

gccacaaacctcttgactcc

5,10-Methylenetetrahydrofolate reductase (Mthfr)

NM_010840

agcttgaagccacctggactgtat

agactagcgttgctgggtttcaga

Glutamylcysteine ligase catalytic subunit (Gclc)

NM_010295

ggaggcgatgttcttgagac

cagagggtcggatggttg

Glutamylcysteine ligase modifier subunit (Gclm)

NM_008129

gactcacaatgacccgaaaga

gatgctttcttgaagagcttcct

Thioredoxin 1 (Trx1)

X77585

cgtggtggacttctctgctacgtggtg

ggtcggcatgcatttgacttcacagtc

Thioredoxin 2 (Trx2)

U85089

gctagagaagatggtcgccaagcagca

tcctcgtccttgatccccacaaacttg

Thioredoxin reductase 1 (TrxR1)

AB027565

ggccaacaaaatcggtgaacacatggaag

cgccagcaacactgtgttaaattcgccct

Thioredoxin reductase 2 (TrxR2)

AB027566

gtcccctcccacatcaaaaaactcccaac

ggcccacaggacagtgtcaaaggtgc

Glutaredoxin 1 (Grx1)

AB013137

tgcagaaagacccaagaaatcctcagtca

tggagattagatcactgcatccgcctatg

Glutaredoxin 2 (Grx2)

NM_023505

catcctgctcttactgttccatggccaa

tcatcttgtgaagcgcatcttgaaactgg

β2-microglobulin (B2M)

NM_00975

atgggaagccgaacatactg

cagtctcagtgggggtgaat