Figure 3
From: Transcriptional regulation of Caenorhabditis elegansFOXO/DAF-16 modulates lifespan
DAF-16d/f and DAF-16a respond differently to changes in insulin/IGF-1 signaling. (A) Lifespan analysis of daf-16(mgDf50); daf-2(e1368) worms carrying daf-16a::gfpHT or daf-16d/f::gfpHT. One of three repeats is shown; each with similar results. All of the lifespan data are shown in Additional file 1: Table S4. (B) Dauer formation in daf-16(mgDf50); daf-2(e1370) or daf-16(mgDf50); daf-2(e1368) worms carrying daf-16a::gfpHTor daf-16d/f::gfpHT at 20°C. The daf-16(mgDf50); daf-2(e1368); daf-16d/f strain did not form dauers, while daf-16(mgDf50); daf-2(e1370); daf-16d/f strain formed a significant fraction of dauers. Dauer formation data represents one experiment with additional repeats showing similar results (Additional file 1: Figure S5). (C) Localization of DAF-16 isoforms in daf-2(e1368) and daf-2(e1370) mutant backgrounds. daf-16(mgDf50); daf-2(e1368) or daf-16(mgDf50); daf-2(e1368) worms carrying daf-16a::gfpHTor daf-16d/f::gfpHT, as well as the daf-2 mutants expressing endogenous daf-16 isoforms, were visualized after incubation at 20°C for 20 hours. Red arrows indicate nuclear DAF-16. (D) Quantification of the nuclear localization of the DAF-16 isoforms in daf-2(e1368) and daf-2(e1370) mutant backgrounds. Nuclear enrichment of DAF-16d/f is observed in daf-2(e1370) worms but not in daf-2(e1368) worms, while nuclear enrichment of DAF-16a is observed in both daf-2 alleles.