Telomere dysfunction induces HOPX , HIST2H2BE , ICEBERG and S100A7 / S100A15 but not the effectors of cell senescence. NHEKs were transduced with TRF2ΔBΔM in three independent experiments resulting in keratinocyte populations expressing TRF2ΔBΔM at different levels: LOW (12-fold), MEDIUM (15-fold) and HIGH (18-fold). Cell extracts were analysed 5 days following expression of the transgene by RT-qPCR for induction of transcript levels of (a) HOPX, HIST2H2BE, ICEBERG and S100A7 (S100A7/S100A15); (b) Cyclin A2 (CCNA2) and Cyclin D1 (CCND1) and (c) effectors of senescence-associated cell cycle arrest p14ARF, p16INK4A and p53. Endogenous TRF2 mRNA levels were also assessed to confirm they remain unaltered upon expression of its dominant-negative mutant TRF2ΔBΔM (TRF2DN). Data are reported as a fold increase in mRNA expression levels relative to the respective empty vector (EV) control. Legend: EV, NHEK expressing empty vector control; DN, NHEK expressing TRF2ΔBΔM.