Figure 4From: The secreted protein S100A7 (psoriasin) is induced by telomere dysfunction in human keratinocytes independently of a DNA damage response and cell cycle regulatorsTranscriptional profile of populations of normal human epidermal keratinocytes expressing p16INK4A. NHEKs were transduced with p16INK4A in three independent experiments resulting in keratinocyte populations expressing p16INK4A at different levels: LOW (18-fold), MEDIUM (60-fold) and HIGH (116-fold). Cell extracts were analysed 5 days following expression of the transgene by RT-qPCR for induction of transcript levels of (a) HOPX, HIST2H2BE, ICEBERG and S100A7 (S100A7/S100A15); (b) Cyclin A2 (CCNA2) and Cyclin D1 (CCND1) and (c) effectors of senescence-associated cell cycle arrest p14ARF, p16INK4A and p53. Data are reported as a fold increase in mRNA expression levels relative to the respective empty vector (EV) control. Legend: EV, NHEK expressing empty vector control; p16, NHEK expressing p16INK4A.Back to article page