Caffeine causes DAF-16 nuclear localization. (A) Treatment with 5 mM caffeine causes nuclear localization of transgenically expressed DAF-16::GFP. A 2-h heat shock at 37°C robustly activates DAF-16 and was used as a positive control. Error bars represent standard error. *P < 0.05 vs. 0 mM. (B) Representative image showing DAF-16::GFP nuclear localization in response to caffeine.