- Open Access
Caffeine extends life span, improves healthspan, and delays age-associated pathology in Caenorhabditis elegans
© Sutphin et al.; licensee BioMed Central Ltd. 2012
- Received: 19 June 2012
- Accepted: 12 September 2012
- Published: 1 December 2012
The longevity of an organism is influenced by both genetic and environmental factors. With respect to genetic factors, a significant effort is being made to identify pharmacological agents that extend life span by targeting pathways with a defined role in the aging process. On the environmental side, the molecular mechanisms responsible for the positive influence of interventions such as dietary restriction are being explored. The environment experienced by humans in modern societies already contains countless compounds that may influence longevity. Understanding the role played by common compounds that substantially affect the aging process will be critical for predicting and interpreting the outcome of introducing new interventions. Caffeine is the most widely used psychoactive drug worldwide. Prior studies in flies, worms, and mice indicate that caffeine may positively impact age-associated neurodegenerative pathology, such as that observed in Alzheimer’s disease.
Here we report that caffeine is capable of extending life span and improving healthspan in Caenorhabditis elegans, a finding that is in agreement with a recently published screen looking for FDA-approved compounds capable of extending worm life span. Life span extension using caffeine displays epistatic interaction with two known longevity interventions: dietary restriction and reduced insulin signaling. Caffeine treatment also delays pathology in a nematode model of polyglutamine disease.
The identification of caffeine as a relevant factor in aging and healthspan in worms, combined with prior work in both humans and rodents linking caffeine consumption to reduced risk of age-associated disease, suggests that caffeine may target conserved longevity pathways. Further, it may be important to consider caffeine consumption when developing clinical interventions, particularly those designed to mimic dietary restriction or modulate insulin/IGF-1-like signaling. The positive impact of caffeine on a worm model of polyglutamine disease suggests that chronic caffeine consumption may generally enhance resistance to proteotoxic stress and may be relevant to assessing risk and developing treatments for human diseases like Alzheimer’s and Huntington’s disease. Future work addressing the relevant targets of caffeine in models of aging and healthspan will help to clarify the underlying mechanisms and potentially identify new molecular targets for disease intervention.
- Life span
Numerous interventions have been identified that extend life span across an evolutionarily diverse range of organisms [1, 2]. These include external (environmental) interventions, such as dietary restriction, heat shock, or treatment with a pharmacological agent, as well as internal (genetic) interventions, such as reduced target of rapamycin (TOR) signaling or reduced insulin/IGF-1-like signaling (IIS). In most cases, studies identifying these interventions are carried out using a genetically homogenous population in a controlled, low-risk, and pathogen-free environment. Interventions that are successful under laboratory conditions are beginning to be introduced into human clinical trials. As these trials progress, it will be important to understand how the artificial nature of the populations and environments in the laboratory setting may impact the outcomes of specific interventions when applied under more variable conditions. In terms of genetics, efforts are underway to understand the effects of dietary restriction in genetically heterogeneous populations. Early evidence indicates that the benefits observed in laboratory populations might not be universally realized by all members of genetically diverse populations [3–7]. From an environmental perspective, human populations are exposed to a wide range of diets, climates, and pharmacological agents that are not present in the laboratory setting. Understanding the impact of these factors on longevity and age-associated disease will be important for predicting unintended effects that might arise from introducing novel interventions.
Caffeine is the most widely used psychoactive substance worldwide. Average consumption in the US is 168 mg/person/day (equivalent to 1 to 2 cups of Starbucks® coffee) and reaches 414 mg/person/day in the Netherlands . Chronic, moderate consumption of caffeine has been linked with reduced risk of age-associated neurodegenerative disorders in humans, including dementia , Alzheimer’s disease [9–11], and Parkinson’s disease [12–15]. In addition, studies performed on elderly human populations have correlated habitual caffeine consumption with reduced mortality [16, 17] and improvements in various measures of healthspan, including reduced cognitive decline [18, 19], improved memory [20–22], and increased motor speed . While other studies failed to find similar correlations, the differences are attributed to wide variation in methodology [23, 24].
The potential for caffeine consumption to reduce neuropathology and impart age-associated neuroprotection has motivated numerous studies using animal models. Research using rodent models of neurodegenerative disease generally demonstrates that caffeine administration can successfully alleviate degenerative symptoms and pathology (review by Cunha and Agostinho ). Acute treatment with caffeine prevents avoidance memory impairment in a rat model of Parkinson's disease , while chronic caffeine treatment prevents cognitive defects in mice expressing toxic forms of amyloid beta, a common rodent model of Alzheimer’s disease . Furthermore, acute caffeine treatment reduces amyloid beta levels in the plasma and brain interstitial fluid  and delays memory defects following intracerebral administration of amyloid beta [29–31]. One study using these mice suggests that caffeine treatment may even restore performance in individuals that are already displaying memory deficits . Neurodegenerative disorders aside, caffeine treatment also prevents memory impairment in rodent models of various other diseases and conditions, including chronic stress, child convulsions, type 1 and type 2 diabetes, attention deficit and hyperactivity disorder, heavy alcohol consumption, and sleep deprivation . Importantly, caffeine improves age-associated memory impairment in both mice and rats [33, 34].
Recently, several groups have started investigating the physiological effects of caffeine in non-mammalian models, including worms and yeast. A common strategy to model Alzheimer’s disease in Caenorhabditis elegans involves transgenic expression of a toxic form of amyloid beta in the body wall muscle, resulting in an age-dependent paralysis phenotype . Dostal et al.  found that both caffeine and non-caffeine components of coffee were capable of delaying paralysis in this model. During the course of the present study, another group identified caffeine in a screen for FDA-approved compounds capable of extending worm life span . In yeast, caffeine increases chronological life span, likely through a mechanism related to TOR signaling .
Given the high consumption rates of caffeine for people living in the developed world, understanding the impact of caffeine on aging and age-related disease will be important as aging interventions move from the laboratory into use in clinical trials and the broader population. In this study, we characterize the effect of caffeine on life span and healthspan in C. elegans and identify clear epistatic interactions between caffeine and both dietary restriction and reduced IIS.
Caffeine extends worm life span in a temperature-dependent manner
Caffeine prolongs mobility and delays polyglutamine-associated pathology
Life span extension by bacterial deprivation and exposure to caffeine are non-additive
Caffeine extends life span in the absence of sir-2.1, hif-1, and cep-1
Caffeine appears to modulate longevity by a mechanism similar to reduced IIS signaling
In this study, we demonstrate that chronic exposure to caffeine during adulthood extends life span and healthspan of C. elegans in a temperature-dependent manner. Life span extension from caffeine is non-additive with life span extension by bacterial deprivation and independent of the hypoxia inducible factor, HIF-1, the C. elegans p53 ortholog, CEP-1, and the C. elegans Sir2 ortholog, SIR-2.1. Caffeine appears to act, at least in part, by activating the FOXO transcription factor DAF-16 in a manner similar to reduced IIS. Chronic caffeine exposure also delays paralysis in a C. elegans model of polyglutamine disease, though this effect appears to be independent of DAF-16.
Perhaps the most intriguing therapeutic value for caffeine is its potential to reduce the risk and delay the onset of age-associated neurodegenerative disease. As described in the introduction, studies in humans and rodents indicate that caffeine treatment reduces the risk of disease onset and improves cognitive decline in models of Alzheimer’s and Parkinson’s disease. Previous work has also shown that caffeine is capable of delaying pathology in worm models of Alzheimer’s disease [36, 37]. In this study, we expand upon these findings to show that caffeine is capable of delaying pathology in a worm model of polyglutamine disease. Although mammalian studies investigating the use of caffeine in neurodegenerative disorders have focused primarily on Alzheimer’s and Parkinson’s disease, research that examines the effects of caffeine consumption on Huntington’s disease in human populations is currently underway. Our finding suggests that a more detailed examination of the influence of caffeine on the progression of a broader spectrum of diseases associated with proteotoxic stress in mammalian models may be warranted.
This study and that by Lublin et al.  both identify an epistatic interaction between caffeine and IIS. A related link has been made in mammals. While acute treatment with caffeine has been shown to increase blood pressure  and reduce insulin sensitivity [53, 54], long-term coffee consumption shows a strong correlation with reduced risk of type 2 diabetes in humans , and chronic caffeine exposure prevents diet-induced insulin resistance and hypertension in rats . Chronic caffeine consumption may prove useful in mimicking reduced IIS and improving diet-induced insulin resistance.
The interaction observed between bacterial deprivation and caffeine suggests that caffeine may also emulate aspects of dietary restriction, which is particularly interesting given that caffeine is already in common use in human society. The observation that caffeine interacts with both bacterial deprivation and IIS is complicated by the fact that bacterial deprivation extends life span independently of both DAF-2 and DAF-16 [46, 48], suggesting that caffeine may activate overlapping downstream targets in both pathways. The additional observation that life span extension from caffeine is reduced in three other strain backgrounds with mutations in genes linked to aging suggests that caffeine may be activating a common set of cellular processes important for increased longevity via a range of interventions. The idea that caffeine may interact with multiple pathways involved in aging is further supported by the observation that the influences of caffeine on life span and polyglutamine toxicity are separable with respect to their dependence on both temperature and DAF-16. Alternatively, it is possible that caffeine alters the molecular or physiological state of the organisms in such a way that renders the organism unable to respond normally to signals that result in increased life span in untreated animals. Epistatic interactions provide limited information regarding the potential mechanism of life span extension , and further study will be required to unravel the complexities of caffeine’s impact on the aging process.
This study identifies genetic interaction between caffeine and both the IIS and the dietary restriction pathways. The direct molecular targets and downstream mechanisms by which caffeine influences longevity remain to be investigated. Mammalian research suggests that caffeine primarily impacts cognitive phenotypes by antagonizing adenosine receptors A1 and A2A. At sub-toxic levels of caffeine, adenosine receptors are the only clear molecular targets and many of the beneficial effects of caffeine are mimicked by specific agonists of adenosine receptor A2A. Mild inhibition of phosphodiesterase activity has only been observed at higher concentrations of caffeine . Functionally, caffeine prevents memory impairment induced by heavy alcohol consumption in rats, an effect that can be mimicked by simultaneous treatment with inhibitors of phosphodiesterase 5 and adenosine receptor A2A (but not by either inhibitor alone) . This suggests that specific subclasses of phosphodiesterases may be important for the action of sub-toxic doses of caffeine in some circumstances.
Clear orthologs of mammalian adenosine receptors have not yet been identified in C. elegans, though there are several candidate genes based on sequence homology. In contrast, the C. elegans genome contains six phosphodiesterases that fall into two functional classes: one class that specifically targets cAMP (PDE-4,6) and another that is thought to target cGMP (PDE-1,2,3,5) [59, 60]. High doses of caffeine have been shown to inhibit mammalian cAMP phosphodiesterases [61, 62], indicating that the former class is of greater interest. Further investigation will determine whether adenosine receptors, phosphodiesterases, or other targets are involved in caffeine’s effect on worm life span.
Two factors have complicated mammalian research with caffeine. In human populations, most studies determine caffeine intake by consumption of caffeine-containing foods and beverages, such as coffee, tea, soft drinks, and chocolate, all of which contain other compounds that have the potential to affect the diseases under investigation. For example, one study found that long-term coffee consumption shows a strong correlation with reduced risk of type 2 diabetes , while a later study found a similar correlation for both caffeinated and decaffeinated coffee consumption . A recently published study identified a correlation between consumption of either caffeinated or decaffeinated coffee and reduced mortality risk for a range of age-associated diseases, including diabetes . The collective findings from these studies suggest that some of the insulin-related benefit from coffee consumption may result from compounds in coffee other than caffeine. Interpretations are further complicated by seemingly contradictory effects resulting from acute and chronic caffeine treatment in some circumstances, as discussed previously with respect to insulin sensitivity and hypertension. C. elegans may be a useful model for decoupling these types of complications. For example, worm studies completed to date have already begun to separate influences from caffeine and non-caffeine sources with respect to coffee. Dostal et al.  identified SKN-1 as a primary downstream factor in the caffeine-independent delay in amyloid beta toxicity using coffee extract, while this study and that by Lublin et al.  identify IIS as an important player in life span extension by caffeine.
A growing accumulation of evidence in humans, rodents, and nematodes suggests that chronic caffeine exposure may yield significant health benefits by delaying aging and preventing specific age-associated pathologies. Recent studies indicate that C. elegans will be a useful system for dismantling the molecular events that underlie the beneficial effects of caffeine. C. elegans research may also help unravel the complications associated with acute versus chronic caffeine treatment and identify other compounds in coffee and tea with the potential to promote longer life span. Overall, based on the observations that caffeine is capable of increasing life span in worms and has been correlated with decreased mortality in humans, we anticipate the expansion of studies examining the influence of caffeine on longevity into mammalian systems.
In this study, we have validated and extended prior evidence that caffeine can improve healthy aging in C. elegans by showing that caffeine can both increase life span and promote resistance to proteotoxic stress. The longevity effects of caffeine appear to overlap with both the insulin-like signaling pathway and with dietary restriction, while the resistance to polyglutamine toxicity is independent of insulin-like signaling. The effects of caffeine on longevity also vary based on the experimental temperature. It will be important to unravel the mechanisms underlying these observations in future studies. In this way, it may be possible to define how caffeine exerts its positive health benefits in worms and determine whether similar processes govern the effects of caffeine on human longevity and healthspan.
Strains and media
The strains used in this study are listed in Additional file 1: Table S2 and were obtained from the Caenorhabditis Genetics Center, the laboratory of Dr. Chris Link (University of Colorado, Boulder, CO, USA), or the laboratory of Dr. Jim Thomas (University of Washington, Seattle, WA, USA). Animals were maintained on solid nematode growth media (NGM) agar plates using standard techniques. Experiments were performed on NGM plates supplemented with 25 mg/ml ampicillin to prevent contamination. Adult worms were placed on NGM plates containing FUdR to prevent reproduction. With the exception of the bacteria used in RNAi experiments, bacterial food was killed by exposure to UV and addition of ampicillin to the medium, as previously described . Solid anhydrous caffeine (MP Biomedicals, Solon, OH, USA) was added directly to the NGM solution prior to autoclaving. Neither autoclaving nor UV treatment during plate preparation influenced life span in the presence of caffeine (data not shown). All experiments were conducted at 15°C except where otherwise noted.
RNAi experiments were conducted using feeding protocols according to standard procedures. The RNAi feeding strains targeting daf-16 and daf-2 were obtained from the Vidal RNAi library  and J. McElwee, respectively. RNAi plasmids were sequenced to verify the target sequence. RNAi plates consisted of NGM supplemented with 1 mM β-D-isothiogalactopyranoside (IPTG) and 25 μg/ml carbenicillin. Worms were raised on RNAi bacteria from egg to the L4 stage of development and then transferred to plates containing freshly seeded RNAi bacteria plus 50 μM FUdR to prevent production.
Life span analysis
Life span experiments were conducted as previously described . Bacterial deprivation was conducted by maintaining animals on UV-killed OP50 bacteria food until day 4 of adulthood and then transferring animals to NGM agar plates containing ampicillin but without a bacterial food source, as previously described [47, 67].
Paralysis of worms was assessed visually as previously described . Worms were scored as paralyzed if they were unable to make forward progress on the NGM surface in response to plate-tapping or tail-prodding.
Quantification of aggregates
Q35::YFP aggregates were quantified by taking images using fluorescent microscopy followed by automated quantification of the number of aggregates present per worm based on pixel density using ImageJ.
Thrashing was quantified visually by suspending individual animals in a droplet of M9 buffer on the surface of an NGM plate and counting the number of body bends in 60 s. Movement rate was quantified by placing an individual worm onto a fresh OP50-seeded NGM plate. After 60 min, pictures were taken of tracks left in the bacterial lawn using a standard SLR camera with a microscope eyepiece adapter. Track length was measured using ImageJ software. Pharyngeal pumping was quantified visually by taking video recordings of the head region of individual animals and counting the number of pumps in 30 s.
DAF-16::GFP nuclear localization
Transgenic worms expressing DAF-16::GFP were transferred to plates containing 0 mM or 5 mM caffeine at the L4 stage of development for. A third population of worms was exposed to 30°C for 2 h immediately prior to analysis. Worms were immobilized by treatment with 25 nM NaN3 and still images captured using a mounted digital camera and the GFP fluorescence channel of a Zeiss SteREO Lumar V.12 microscope. Captured images were used to quantify visible GFP foci.
Statistical significance was determined for worm life span using the Wilcoxon rank sum test. All other comparisons were conducted using an unpaired two-tailed Student’s T-test assuming unequal variance.
We thank Dr. Jennifer Schleit for helpful discussion during the conception and experimental design of this study. This work was supported by NIH Grant R01AG031108 and R01AG038518 to MK. GLS and MEY were supported by NIH Training Grant T32AG000057. MK is an Ellison Medical Foundation New Scholar in Aging.
- Fontana L, Partridge L, Longo VD: Extending healthy life span–from yeast to humans. Science. 2010, 328: 321-326. 10.1126/science.1172539.PubMed CentralView ArticlePubMedGoogle Scholar
- Kenyon CJ: The genetics of ageing. Nature. 2010, 464: 504-512. 10.1038/nature08980.View ArticlePubMedGoogle Scholar
- Liao CY, Rikke BA, Johnson TE, Diaz V, Nelson JF: Genetic variation in the murine lifespan response to dietary restriction: from life extension to life shortening. Aging Cell. 2010, 9: 92-95. 10.1111/j.1474-9726.2009.00533.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Liao CY, Rikke BA, Johnson TE, Gelfond JA, Diaz V, Nelson JF: Fat maintenance is a predictor of the murine lifespan response to dietary restriction. Aging Cell. 2011, 10: 629-639. 10.1111/j.1474-9726.2011.00702.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Rikke BA, Liao CY, McQueen MB, Nelson JF, Johnson TE: Genetic dissection of dietary restriction in mice supports the metabolic efficiency model of life extension. Exp Gerontol. 2010, 45: 691-701. 10.1016/j.exger.2010.04.008.PubMed CentralView ArticlePubMedGoogle Scholar
- Schleit J, Wasko BM, Kaeberlein M: Yeast as a model to understand the interaction between genotype and the response to calorie restriction. FEBS Lett. 2012, 586: 2868-2873. 10.1016/j.febslet.2012.07.038.PubMed CentralView ArticlePubMedGoogle Scholar
- Harper JM, Leathers CW, Austad SN: Does caloric restriction extend life in wild mice?. Aging Cell. 2006, 5: 441-449. 10.1111/j.1474-9726.2006.00236.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Fredholm BB, Battig K, Holmen J, Nehlig A, Zvartau EE: Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacol Rev. 1999, 51: 83-133.PubMedGoogle Scholar
- Eskelinen MH, Kivipelto M: Caffeine as a protective factor in dementia and Alzheimer's disease. J Alzheimers Dis. 2010, 20 (Suppl 1): S167-S174.PubMedGoogle Scholar
- Lindsay J, Laurin D, Verreault R, Hebert R, Helliwell B, Hill GB, McDowell I: Risk factors for Alzheimer's disease: a prospective analysis from the Canadian study of health and aging. Am J Epidemiol. 2002, 156: 445-453. 10.1093/aje/kwf074.View ArticlePubMedGoogle Scholar
- Maia L, de Mendonca A: Does caffeine intake protect from Alzheimer's disease?. Eur J Neurol. 2002, 9: 377-382. 10.1046/j.1468-1331.2002.00421.x.View ArticlePubMedGoogle Scholar
- Ascherio A, Zhang SM, Hernan MA, Kawachi I, Colditz GA, Speizer FE, Willett WC: Prospective study of caffeine consumption and risk of Parkinson's disease in men and women. Ann Neurol. 2001, 50: 56-63. 10.1002/ana.1052.View ArticlePubMedGoogle Scholar
- Benedetti MD, Bower JH, Maraganore DM, McDonnell SK, Peterson BJ, Ahlskog JE, Schaid DJ, Rocca WA: Smoking, alcohol, and coffee consumption preceding Parkinson's disease: a case–control study. Neurology. 2000, 55: 1350-1358. 10.1212/WNL.55.9.1350.View ArticlePubMedGoogle Scholar
- Costa J, Lunet N, Santos C, Santos J, Vaz-Carneiro A: Caffeine exposure and the risk of Parkinson's disease: a systematic review and meta-analysis of observational studies. J Alzheimers Dis. 2010, 20 (Suppl 1): S221-S238.PubMedGoogle Scholar
- Ross GW, Abbott RD, Petrovitch H, Morens DM, Grandinetti A, Tung KH, Tanner CM, Masaki KH, Blanchette PL, Curb JD, et al: Association of coffee and caffeine intake with the risk of Parkinson disease. Jama. 2000, 283: 2674-2679. 10.1001/jama.283.20.2674.View ArticlePubMedGoogle Scholar
- Fortes C, Forastiere F, Farchi S, Rapiti E, Pastori G, Perucci CA: Diet and overall survival in a cohort of very elderly people. Epidemiology. 2000, 11: 440-445. 10.1097/00001648-200007000-00013.View ArticlePubMedGoogle Scholar
- Paganini-Hill A, Kawas CH, Corrada MM: Non-alcoholic beverage and caffeine consumption and mortality: the leisure world cohort study. Prev Med. 2007, 44: 305-310. 10.1016/j.ypmed.2006.12.011.PubMed CentralView ArticlePubMedGoogle Scholar
- Santos C, Lunet N, Azevedo A, de Mendonca A, Ritchie K, Barros H: Caffeine intake is associated with a lower risk of cognitive decline: a cohort study from Portugal. J Alzheimers Dis. 2010, 20 (Suppl 1): S175-S185.PubMedGoogle Scholar
- van Gelder BM, Buijsse B, Tijhuis M, Kalmijn S, Giampaoli S, Nissinen A, Kromhout D: Coffee consumption is inversely associated with cognitive decline in elderly European men: the FINE Study. Eur J Clin Nutr. 2007, 61: 226-232. 10.1038/sj.ejcn.1602495.View ArticlePubMedGoogle Scholar
- Hameleers PA, Van Boxtel MP, Hogervorst E, Riedel WJ, Houx PJ, Buntinx F, Jolles J: Habitual caffeine consumption and its relation to memory, attention, planning capacity and psychomotor performance across multiple age groups. Hum Psychopharmacol. 2000, 15: 573-581. 10.1002/hup.218.View ArticlePubMedGoogle Scholar
- Johnson-Kozlow M, Kritz-Silverstein D, Barrett-Connor E, Morton D: Coffee consumption and cognitive function among older adults. Am J Epidemiol. 2002, 156: 842-850. 10.1093/aje/kwf119.View ArticlePubMedGoogle Scholar
- Ritchie K, Carriere I, de Mendonca A, Portet F, Dartigues JF, Rouaud O, Barberger-Gateau P, Ancelin ML: The neuroprotective effects of caffeine: a prospective population study (the Three City Study). Neurology. 2007, 69: 536-545. 10.1212/01.wnl.0000266670.35219.0c.View ArticlePubMedGoogle Scholar
- Rosso A, Mossey J, Lippa CF: Caffeine: neuroprotective functions in cognition and Alzheimer's disease. Am J Alzheimers Dis Other Demen. 2008, 23: 417-422. 10.1177/1533317508320083.View ArticlePubMedGoogle Scholar
- Santos C, Costa J, Santos J, Vaz-Carneiro A, Lunet N: Caffeine intake and dementia: systematic review and meta-analysis. J Alzheimers Dis. 2010, 20 (Suppl 1): S187-S204.PubMedGoogle Scholar
- Cunha RA, Agostinho PM: Chronic caffeine consumption prevents memory disturbance in different animal models of memory decline. J Alzheimers Dis. 2010, 20 (Suppl 1): S95-S116.PubMedGoogle Scholar
- Gevaerd MS, Takahashi RN, Silveira R, Da Cunha C: Caffeine reverses the memory disruption induced by intra-nigral MPTP-injection in rats. Brain Res Bull. 2001, 55: 101-106. 10.1016/S0361-9230(01)00501-9.View ArticlePubMedGoogle Scholar
- Arendash GW, Schleif W, Rezai-Zadeh K, Jackson EK, Zacharia LC, Cracchiolo JR, Shippy D, Tan J: Caffeine protects Alzheimer's mice against cognitive impairment and reduces brain beta-amyloid production. Neuroscience. 2006, 142: 941-952. 10.1016/j.neuroscience.2006.07.021.View ArticlePubMedGoogle Scholar
- Cao C, Cirrito JR, Lin X, Wang L, Verges DK, Dickson A, Mamcarz M, Zhang C, Mori T, Arendash GW, et al: Caffeine suppresses amyloid-beta levels in plasma and brain of Alzheimer's disease transgenic mice. J Alzheimers Dis. 2009, 17: 681-697.PubMed CentralPubMedGoogle Scholar
- Canas PM, Porciuncula LO, Cunha GM, Silva CG, Machado NJ, Oliveira JM, Oliveira CR, Cunha RA: Adenosine A2A receptor blockade prevents synaptotoxicity and memory dysfunction caused by beta-amyloid peptides via p38 mitogen-activated protein kinase pathway. J Neurosci. 2009, 29: 14741-14751. 10.1523/JNEUROSCI.3728-09.2009.View ArticlePubMedGoogle Scholar
- Cunha GM, Canas PM, Melo CS, Hockemeyer J, Muller CE, Oliveira CR, Cunha RA: Adenosine A2A receptor blockade prevents memory dysfunction caused by beta-amyloid peptides but not by scopolamine or MK-801. Exp Neurol. 2008, 210: 776-781. 10.1016/j.expneurol.2007.11.013.View ArticlePubMedGoogle Scholar
- Dall'Igna OP, Fett P, Gomes MW, Souza DO, Cunha RA, Lara DR: Caffeine and adenosine A(2a) receptor antagonists prevent beta-amyloid (25–35)-induced cognitive deficits in mice. Exp Neurol. 2007, 203: 241-245. 10.1016/j.expneurol.2006.08.008.View ArticlePubMedGoogle Scholar
- Arendash GW, Mori T, Cao C, Mamcarz M, Runfeldt M, Dickson A, Rezai-Zadeh K, Tane J, Citron BA, Lin X, et al: Caffeine reverses cognitive impairment and decreases brain amyloid-beta levels in aged Alzheimer's disease mice. J Alzheimers Dis. 2009, 17: 661-680.PubMedGoogle Scholar
- Costa MS, Botton PH, Mioranzza S, Souza DO, Porciuncula LO: Caffeine prevents age-associated recognition memory decline and changes brain-derived neurotrophic factor and tirosine kinase receptor (TrkB) content in mice. Neuroscience. 2008, 153: 1071-1078. 10.1016/j.neuroscience.2008.03.038.View ArticlePubMedGoogle Scholar
- Prediger RD, Batista LC, Takahashi RN: Caffeine reverses age-related deficits in olfactory discrimination and social recognition memory in rats. Involvement of adenosine A1 and A2A receptors. Neurobiol Aging. 2005, 26: 957-964. 10.1016/j.neurobiolaging.2004.08.012.View ArticlePubMedGoogle Scholar
- Link CD, Taft A, Kapulkin V, Duke K, Kim S, Fei Q, Wood DE, Sahagan BG: Gene expression analysis in a transgenic Caenorhabditis elegans Alzheimer's disease model. Neurobiol Aging. 2003, 24: 397-413. 10.1016/S0197-4580(02)00224-5.View ArticlePubMedGoogle Scholar
- Dostal V, Roberts CM, Link CD: Genetic mechanisms of coffee extract protection in a Caenorhabditis elegans model of beta-amyloid peptide toxicity. Genetics. 2010, 186: 857-866. 10.1534/genetics.110.120436.PubMed CentralView ArticlePubMedGoogle Scholar
- Lublin A, Isoda F, Patel H, Yen K, Nguyen L, Hajje D, Schwartz M, Mobbs C: FDA-approved drugs that protect mammalian neurons from glucose toxicity slow aging dependent on cbp and protect against proteotoxicity. PLoS One. 2011, 6: e27762-10.1371/journal.pone.0027762.PubMed CentralView ArticlePubMedGoogle Scholar
- Wanke V, Cameroni E, Uotila A, Piccolis M, Urban J, Loewith R, De Virgilio C: Caffeine extends yeast lifespan by targeting TORC1. Mol Microbiol. 2008, 69: 277-285. 10.1111/j.1365-2958.2008.06292.x.View ArticlePubMedGoogle Scholar
- Leiser SF, Begun A, Kaeberlein M: HIF-1 modulates longevity and healthspan in a temperature-dependent manner. Aging Cell. 2011, 10: 318-326. 10.1111/j.1474-9726.2011.00672.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Kuranda K, Leberre V, Sokol S, Palamarczyk G, Francois J: Investigating the caffeine effects in the yeast Saccharomyces cerevisiae brings new insights into the connection between TOR, PKC and Ras/cAMP signalling pathways. Mol Microbiol. 2006, 61: 1147-1166. 10.1111/j.1365-2958.2006.05300.x.View ArticlePubMedGoogle Scholar
- Sun J, Kale SP, Childress AM, Pinswasdi C, Jazwinski SM: Divergent roles of RAS1 and RAS2 in yeast longevity. J Biol Chem. 1994, 269: 18638-18645.PubMedGoogle Scholar
- Link CD: Expression of human beta-amyloid peptide in transgenic Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1995, 92: 9368-9372. 10.1073/pnas.92.20.9368.PubMed CentralView ArticlePubMedGoogle Scholar
- Bonini NM, La Spada AR: Silencing polyglutamine degeneration with RNAi. Neuron. 2005, 48: 715-718. 10.1016/j.neuron.2005.11.008.View ArticlePubMedGoogle Scholar
- Kaeberlein M: Resveratrol and rapamycin: are they anti-aging drugs?. Bioessays. 2010, 32: 96-99. 10.1002/bies.200900171.View ArticlePubMedGoogle Scholar
- Ingram DK, Zhu M, Mamczarz J, Zou S, Lane MA, Roth GS, DeCabo R: Calorie restriction mimetics: an emerging research field. Aging Cell. 2006, 5: 97-108. 10.1111/j.1474-9726.2006.00202.x.View ArticlePubMedGoogle Scholar
- Kaeberlein TL, Smith ED, Tsuchiya M, Welton KL, Thomas JH, Fields S, Kennedy BK, Kaeberlein M: Lifespan extension in Caenorhabditis elegans by complete removal of food. Aging Cell. 2006, 5: 487-494. 10.1111/j.1474-9726.2006.00238.x.View ArticlePubMedGoogle Scholar
- Smith ED, Kaeberlein TL, Lydum BT, Sager J, Welton KL, Kennedy BK, Kaeberlein M: Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegans. BMC Dev Biol. 2008, 8: 49-10.1186/1471-213X-8-49.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee GD, Wilson MA, Zhu M, Wolkow CA, de Cabo R, Ingram DK, Zou S: Dietary deprivation extends lifespan in Caenorhabditis elegans. Aging Cell. 2006, 5: 515-524. 10.1111/j.1474-9726.2006.00241.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Avery L: The genetics of feeding in Caenorhabditis elegans. Genetics. 1993, 133: 897-917.PubMed CentralPubMedGoogle Scholar
- Lakowski B, Hekimi S: The genetics of caloric restriction in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1998, 95: 13091-13096. 10.1073/pnas.95.22.13091.PubMed CentralView ArticlePubMedGoogle Scholar
- Mehta R, Steinkraus KA, Sutphin GL, Ramos FJ, Shamieh LS, Huh A, Davis C, Chandler-Brown D, Kaeberlein M: Proteasomal regulation of the hypoxic response modulates aging in C. elegans. Science. 2009, 324: 1196-1198. 10.1126/science.1173507.PubMed CentralView ArticlePubMedGoogle Scholar
- Riksen NP, Rongen GA, Smits P: Acute and long-term cardiovascular effects of coffee: implications for coronary heart disease. Pharmacol Ther. 2009, 121: 185-191. 10.1016/j.pharmthera.2008.10.006.View ArticlePubMedGoogle Scholar
- Keijzers GB, De Galan BE, Tack CJ, Smits P: Caffeine can decrease insulin sensitivity in humans. Diabetes Care. 2002, 25: 364-369. 10.2337/diacare.25.2.364.View ArticlePubMedGoogle Scholar
- Moisey LL, Kacker S, Bickerton AC, Robinson LE, Graham TE: Caffeinated coffee consumption impairs blood glucose homeostasis in response to high and low glycemic index meals in healthy men. Am J Clin Nutr. 2008, 87: 1254-1261.PubMedGoogle Scholar
- van Dam RM, Hu FB: Coffee consumption and risk of type 2 diabetes: a systematic review. Jama. 2005, 294: 97-104. 10.1001/jama.294.1.97.View ArticlePubMedGoogle Scholar
- Conde SV, Nunes da Silva T, Gonzalez C, Mota Carmo M, Monteiro EC, Guarino MP: Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats. Br J Nutr. 2012, 107: 86-95. 10.1017/S0007114511002406.View ArticlePubMedGoogle Scholar
- Delaney JR, Sutphin GL, Dulken B, Sim S, Kim JR, Robison B, Schleit J, Murakami CJ, Carr D, An EH, et al: Sir2 deletion prevents lifespan extension in 32 long-lived mutants. Aging Cell. 2011, 10: 1089-1091. 10.1111/j.1474-9726.2011.00742.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Spinetta MJ, Woodlee MT, Feinberg LM, Stroud C, Schallert K, Cormack LK, Schallert T: Alcohol-induced retrograde memory impairment in rats: prevention by caffeine. Psychopharmacology (Berl). 2008, 201: 361-371. 10.1007/s00213-008-1294-5.View ArticleGoogle Scholar
- Liu J, Ward A, Gao J, Dong Y, Nishio N, Inada H, Kang L, Yu Y, Ma D, Xu T, et al: C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog. Nat Neurosci. 2010, 13: 715-722. 10.1038/nn.2540.PubMed CentralView ArticlePubMedGoogle Scholar
- Omori K, Kotera J: Overview of PDEs and their regulation. Circ Res. 2007, 100: 309-327. 10.1161/01.RES.0000256354.95791.f1.View ArticlePubMedGoogle Scholar
- Butcher FR, Potter VR: Control of the adenosine 3',5'-monophosphate-adenyl cyclase system in the livers of developing rats. Cancer Res. 1972, 32: 2141-2147.PubMedGoogle Scholar
- Tsuzuki J, Newburgh RW: Inhibition of 5'-nucleotidase in rat brain by methylxanthines. J Neurochem. 1975, 25: 895-896. 10.1111/j.1471-4159.1975.tb04424.x.View ArticlePubMedGoogle Scholar
- van Dam RM, Willett WC, Manson JE, Hu FB: Coffee, caffeine, and risk of type 2 diabetes: a prospective cohort study in younger and middle-aged U.S. women. Diabetes Care. 2006, 29: 398-403. 10.2337/diacare.29.02.06.dc05-1512.View ArticlePubMedGoogle Scholar
- Freedman ND, Park Y, Abnet CC, Hollenbeck AR, Sinha R: Association of coffee drinking with total and cause-specific mortality. N Engl J Med. 2012, 366: 1891-1904. 10.1056/NEJMoa1112010.PubMed CentralView ArticlePubMedGoogle Scholar
- Sutphin GL, Kaeberlein M: Measuring Caenorhabditis elegans life span on solid media. J Vis Exp. 2009, 27: e1152-Google Scholar
- Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, Hirozane-Kishikawa T, Vandenhaute J, Orkin SH, Hill DE, van den Heuvel S, Vidal M: Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 2004, 14: 2162-2168. 10.1101/gr.2505604.PubMed CentralView ArticlePubMedGoogle Scholar
- Schleit J, Wall VZ, Simko M, Kaeberlein M: The MDT-15 subunit of mediator interacts with dietary restriction to modulate longevity and fluoranthene toxicity in Caenorhabditis elegans. PLoS One. 2011, 6: e28036-10.1371/journal.pone.0028036.PubMed CentralView ArticlePubMedGoogle Scholar
- Steinkraus KA, Smith ED, Davis C, Carr D, Pendergrass WR, Sutphin GL, Kennedy BK, Kaeberlein M: Dietary restriction suppresses proteotoxicity and enhances longevity by an hsf-1-dependent mechanism in Caenorhabditis elegans. Aging Cell. 2008, 7: 394-404. 10.1111/j.1474-9726.2008.00385.x.PubMed CentralView ArticlePubMedGoogle Scholar
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